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1x phosphate buffered saline solution  (Thermo Fisher)


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    Thermo Fisher 1x phosphate buffered saline solution
    1x Phosphate Buffered Saline Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+phosphate+buffered+saline+solution/Phosphate-buffered+saline+(PBS%2C+1X)%2C+sterile-filtered/bio_rxiv__64898__2026__04__16__718897-80-6-11
    Average 99 stars, based on 1 article reviews
    1x phosphate buffered saline solution - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Saline:

    Article Title: The molecular basis for acetylhistidine synthesis by HisAT/NAT16.
    Article Snippet: Forty-eight hours post transfection, the cells were prepped for single cell sorting into three 96 well plates (Sarstedt #83.3924.500) containing 200 μl 20% FBS, 1 % Penicillin-Streptomycin, IMDM cell medium. .. They were trypsinated (Gibco, # 25300054), washed in 1x Phosphate buffered saline solution (PBS, Gibco, #18912014, 1xPBS), harvested at 300 x g for 5min, and finally resuspended in roughly 3ml IMDM. .. EGFP- and mCherry-containing cells were detected with filter FL1 (525/50) and FL3 (617/30), respectively and single sorted on a Sony SH800 cell sorter using Flowjo software at the Bergen Flow Cytometry Core Facility (Supplementary Fig. 13).

    Article Title: The Immunophenotype and Proviral Landscape of HIV-infected CD4 T Cells During Antiretroviral Therapy
    Article Snippet: .. On the 5 th wash, supernatant was aspirated and the sample was resuspended in 200 μL Cell Resuspension Buffer, consisting of 18% OptiPrep Density Gradient Medium (Sigma-Aldrich D1556) and 0.03% filtered Pluronic F-68 (Gibco 24040-032) in filtered 1x Phosphate Buffered Saline Solution (Gibco 10010-023). ..

    Article Title: Comparative Evaluation of Carbohydrate, Amino Acid, and Ionic Liquid Excipients for Flavivirus Vaccine Stabilization
    Article Snippet: The inoculum was aspirated and overlaid with 2X Modified Eagle's Medium (MEM) (MEM; Gibco, Cat. 11-935-046, Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) fetal https://doi.org/10.1021/acsomega.6c00106 ACS Omega XXXX, XXX, XXX−XXX C bovine serum, 2 mM Glutamax, 100 U·mL−1 Penicillin G and 100 μg· mL−1 Streptomycin, 0.25 μg·mL−1 amphotericin B and microcrystalline cellulose Avicel solution 1.2% (w/v) (FMC BioPolymer, Philadelphia, PA). .. The infected cells were incubated at 37 °C with 5% (v/v) CO2 for 72 h. After which the overlay was aspirated and infected cultures were fixed with 10% (w/v) formalin (FormaldeFresh Solution, Buffered, Fisher Chemical, Cat. SF93-4, Thermo Fisher Scientific, Inc.) overnight at 4 °C, permeabilized with 70% (w/ v) pure ethanol for 20 min minimum, and rinsed with 1X phosphate buffered saline solution, pH 7.4 (PBS, Fisher BioReagents Cat. BP665-1, Thermo Fisher Scientific) prior to immunostaining. .. The virus foci were immunostained using human monoclonal antibodies (hMAbs) 1.6D and D11C as previously described28 as primary tags in a 1X PBS solution with 0.1% (v/v) Tween 20 (Tween 20; Cat. 156054, Thermo Fisher Scientific Inc.), 5% (w/v) nonfat dry milk, and final antibody concentration of 1 μg/mL,28 rocked overnight at room temperature.

    Article Title: Outcomes of autologous bone marrow mononuclear cell administration combined with educational intervention in the treatment of autism spectrum disorder: a randomized, open-label, controlled phase II clinical trial
    Article Snippet: .. In brief, the cells were isolated using density gradient centrifugation with Ficoll-PaqueTM PREMIUM density gradient media (Cytiva, Sweden) at 1400xg for 18 min, washed with 1x phosphate-buffered saline solution (Gibco, USA), and resuspended in 10 ml of autologous plasma. .. The total blood components before and after Ficoll‐Paque separation were evaluated by the laboratory department using a whole blood count.

    Article Title: The molecular basis for acetylhistidine synthesis by HisAT/NAT16
    Article Snippet: Forty-eight hours post transfection, the cells were prepped for single cell sorting into three 96 well plates (Sarstedt #83.3924.500) containing 200 μl 20 % FBS, 1 % Penicillin-Streptomycin, IMDM cell medium. .. They were trypsinated (Gibco, # 25300054), washed in 1x Phosphate buffered saline solution (PBS, Gibco, #18912014, 1xPBS), harvested at 300 x g for 5 min, and finally resuspended in roughly 3 ml IMDM. .. EGFP- and mCherry-containing cells were detected with filter FL1 (525/50) and FL3 (617/30), respectively and single sorted on a Sony SH800 cell sorter using Flowjo software at the Bergen Flow Cytometry Core Facility (Supplementary Fig. ).

    Article Title: An Alternative DNA Endonuclease Activity is Associated with the LINE-1 ORF2-encoded Protein
    Article Snippet: On days 2-5, the medium was replaced daily with DMEM-complete medium containing 200 μg/mL hygromycin B (Gibco). .. On day 5, the cells were collected, washed with cold 1x phosphate buffered saline solution (PBS, Gibco), and flash-frozen in a dry ice/ethanol bath. ..

    Article Title: Comparative Evaluation of Carbohydrate, Amino Acid, and Ionic Liquid Excipients for Flavivirus Vaccine Stabilization
    Article Snippet: The inoculum was aspirated and overlaid with 2X Modified Eagle's Medium (MEM) (MEM; Gibco, Cat. 11-935-046, Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) fetal bovine serum, 2 mM Glutamax, 100 U·mL –1 Penicillin G and 100 μg·mL –1 Streptomycin, 0.25 μg·mL –1 amphotericin B and microcrystalline cellulose Avicel solution 1.2% (w/v) (FMC BioPolymer, Philadelphia, PA). .. The infected cells were incubated at 37 °C with 5% (v/v) CO 2 for 72 h. After which the overlay was aspirated and infected cultures were fixed with 10% (w/v) formalin (Formalde-Fresh Solution, Buffered, Fisher Chemical, Cat. SF93-4, Thermo Fisher Scientific, Inc.) overnight at 4 °C, permeabilized with 70% (w/v) pure ethanol for 20 min minimum, and rinsed with 1X phosphate buffered saline solution, pH 7.4 (PBS, Fisher BioReagents Cat. BP665-1, Thermo Fisher Scientific) prior to immunostaining. .. The virus foci were immunostained using human monoclonal antibodies (hMAbs) 1.6D and D11C as previously described as primary tags in a 1X PBS solution with 0.1% (v/v) Tween 20 (Tween 20; Cat. 156054, Thermo Fisher Scientific Inc.), 5% (w/v) nonfat dry milk, and final antibody concentration of 1 μg/mL, rocked overnight at room temperature.

    Infection:

    Article Title: Comparative Evaluation of Carbohydrate, Amino Acid, and Ionic Liquid Excipients for Flavivirus Vaccine Stabilization
    Article Snippet: The inoculum was aspirated and overlaid with 2X Modified Eagle's Medium (MEM) (MEM; Gibco, Cat. 11-935-046, Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) fetal https://doi.org/10.1021/acsomega.6c00106 ACS Omega XXXX, XXX, XXX−XXX C bovine serum, 2 mM Glutamax, 100 U·mL−1 Penicillin G and 100 μg· mL−1 Streptomycin, 0.25 μg·mL−1 amphotericin B and microcrystalline cellulose Avicel solution 1.2% (w/v) (FMC BioPolymer, Philadelphia, PA). .. The infected cells were incubated at 37 °C with 5% (v/v) CO2 for 72 h. After which the overlay was aspirated and infected cultures were fixed with 10% (w/v) formalin (FormaldeFresh Solution, Buffered, Fisher Chemical, Cat. SF93-4, Thermo Fisher Scientific, Inc.) overnight at 4 °C, permeabilized with 70% (w/ v) pure ethanol for 20 min minimum, and rinsed with 1X phosphate buffered saline solution, pH 7.4 (PBS, Fisher BioReagents Cat. BP665-1, Thermo Fisher Scientific) prior to immunostaining. .. The virus foci were immunostained using human monoclonal antibodies (hMAbs) 1.6D and D11C as previously described28 as primary tags in a 1X PBS solution with 0.1% (v/v) Tween 20 (Tween 20; Cat. 156054, Thermo Fisher Scientific Inc.), 5% (w/v) nonfat dry milk, and final antibody concentration of 1 μg/mL,28 rocked overnight at room temperature.

    Article Title: Comparative Evaluation of Carbohydrate, Amino Acid, and Ionic Liquid Excipients for Flavivirus Vaccine Stabilization
    Article Snippet: The inoculum was aspirated and overlaid with 2X Modified Eagle's Medium (MEM) (MEM; Gibco, Cat. 11-935-046, Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) fetal bovine serum, 2 mM Glutamax, 100 U·mL –1 Penicillin G and 100 μg·mL –1 Streptomycin, 0.25 μg·mL –1 amphotericin B and microcrystalline cellulose Avicel solution 1.2% (w/v) (FMC BioPolymer, Philadelphia, PA). .. The infected cells were incubated at 37 °C with 5% (v/v) CO 2 for 72 h. After which the overlay was aspirated and infected cultures were fixed with 10% (w/v) formalin (Formalde-Fresh Solution, Buffered, Fisher Chemical, Cat. SF93-4, Thermo Fisher Scientific, Inc.) overnight at 4 °C, permeabilized with 70% (w/v) pure ethanol for 20 min minimum, and rinsed with 1X phosphate buffered saline solution, pH 7.4 (PBS, Fisher BioReagents Cat. BP665-1, Thermo Fisher Scientific) prior to immunostaining. .. The virus foci were immunostained using human monoclonal antibodies (hMAbs) 1.6D and D11C as previously described as primary tags in a 1X PBS solution with 0.1% (v/v) Tween 20 (Tween 20; Cat. 156054, Thermo Fisher Scientific Inc.), 5% (w/v) nonfat dry milk, and final antibody concentration of 1 μg/mL, rocked overnight at room temperature.

    Incubation:

    Article Title: Comparative Evaluation of Carbohydrate, Amino Acid, and Ionic Liquid Excipients for Flavivirus Vaccine Stabilization
    Article Snippet: The inoculum was aspirated and overlaid with 2X Modified Eagle's Medium (MEM) (MEM; Gibco, Cat. 11-935-046, Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) fetal https://doi.org/10.1021/acsomega.6c00106 ACS Omega XXXX, XXX, XXX−XXX C bovine serum, 2 mM Glutamax, 100 U·mL−1 Penicillin G and 100 μg· mL−1 Streptomycin, 0.25 μg·mL−1 amphotericin B and microcrystalline cellulose Avicel solution 1.2% (w/v) (FMC BioPolymer, Philadelphia, PA). .. The infected cells were incubated at 37 °C with 5% (v/v) CO2 for 72 h. After which the overlay was aspirated and infected cultures were fixed with 10% (w/v) formalin (FormaldeFresh Solution, Buffered, Fisher Chemical, Cat. SF93-4, Thermo Fisher Scientific, Inc.) overnight at 4 °C, permeabilized with 70% (w/ v) pure ethanol for 20 min minimum, and rinsed with 1X phosphate buffered saline solution, pH 7.4 (PBS, Fisher BioReagents Cat. BP665-1, Thermo Fisher Scientific) prior to immunostaining. .. The virus foci were immunostained using human monoclonal antibodies (hMAbs) 1.6D and D11C as previously described28 as primary tags in a 1X PBS solution with 0.1% (v/v) Tween 20 (Tween 20; Cat. 156054, Thermo Fisher Scientific Inc.), 5% (w/v) nonfat dry milk, and final antibody concentration of 1 μg/mL,28 rocked overnight at room temperature.

    Article Title: Comparative Evaluation of Carbohydrate, Amino Acid, and Ionic Liquid Excipients for Flavivirus Vaccine Stabilization
    Article Snippet: The inoculum was aspirated and overlaid with 2X Modified Eagle's Medium (MEM) (MEM; Gibco, Cat. 11-935-046, Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) fetal bovine serum, 2 mM Glutamax, 100 U·mL –1 Penicillin G and 100 μg·mL –1 Streptomycin, 0.25 μg·mL –1 amphotericin B and microcrystalline cellulose Avicel solution 1.2% (w/v) (FMC BioPolymer, Philadelphia, PA). .. The infected cells were incubated at 37 °C with 5% (v/v) CO 2 for 72 h. After which the overlay was aspirated and infected cultures were fixed with 10% (w/v) formalin (Formalde-Fresh Solution, Buffered, Fisher Chemical, Cat. SF93-4, Thermo Fisher Scientific, Inc.) overnight at 4 °C, permeabilized with 70% (w/v) pure ethanol for 20 min minimum, and rinsed with 1X phosphate buffered saline solution, pH 7.4 (PBS, Fisher BioReagents Cat. BP665-1, Thermo Fisher Scientific) prior to immunostaining. .. The virus foci were immunostained using human monoclonal antibodies (hMAbs) 1.6D and D11C as previously described as primary tags in a 1X PBS solution with 0.1% (v/v) Tween 20 (Tween 20; Cat. 156054, Thermo Fisher Scientific Inc.), 5% (w/v) nonfat dry milk, and final antibody concentration of 1 μg/mL, rocked overnight at room temperature.

    Immunostaining:

    Article Title: Comparative Evaluation of Carbohydrate, Amino Acid, and Ionic Liquid Excipients for Flavivirus Vaccine Stabilization
    Article Snippet: The inoculum was aspirated and overlaid with 2X Modified Eagle's Medium (MEM) (MEM; Gibco, Cat. 11-935-046, Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) fetal https://doi.org/10.1021/acsomega.6c00106 ACS Omega XXXX, XXX, XXX−XXX C bovine serum, 2 mM Glutamax, 100 U·mL−1 Penicillin G and 100 μg· mL−1 Streptomycin, 0.25 μg·mL−1 amphotericin B and microcrystalline cellulose Avicel solution 1.2% (w/v) (FMC BioPolymer, Philadelphia, PA). .. The infected cells were incubated at 37 °C with 5% (v/v) CO2 for 72 h. After which the overlay was aspirated and infected cultures were fixed with 10% (w/v) formalin (FormaldeFresh Solution, Buffered, Fisher Chemical, Cat. SF93-4, Thermo Fisher Scientific, Inc.) overnight at 4 °C, permeabilized with 70% (w/ v) pure ethanol for 20 min minimum, and rinsed with 1X phosphate buffered saline solution, pH 7.4 (PBS, Fisher BioReagents Cat. BP665-1, Thermo Fisher Scientific) prior to immunostaining. .. The virus foci were immunostained using human monoclonal antibodies (hMAbs) 1.6D and D11C as previously described28 as primary tags in a 1X PBS solution with 0.1% (v/v) Tween 20 (Tween 20; Cat. 156054, Thermo Fisher Scientific Inc.), 5% (w/v) nonfat dry milk, and final antibody concentration of 1 μg/mL,28 rocked overnight at room temperature.

    Article Title: Comparative Evaluation of Carbohydrate, Amino Acid, and Ionic Liquid Excipients for Flavivirus Vaccine Stabilization
    Article Snippet: The inoculum was aspirated and overlaid with 2X Modified Eagle's Medium (MEM) (MEM; Gibco, Cat. 11-935-046, Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) fetal bovine serum, 2 mM Glutamax, 100 U·mL –1 Penicillin G and 100 μg·mL –1 Streptomycin, 0.25 μg·mL –1 amphotericin B and microcrystalline cellulose Avicel solution 1.2% (w/v) (FMC BioPolymer, Philadelphia, PA). .. The infected cells were incubated at 37 °C with 5% (v/v) CO 2 for 72 h. After which the overlay was aspirated and infected cultures were fixed with 10% (w/v) formalin (Formalde-Fresh Solution, Buffered, Fisher Chemical, Cat. SF93-4, Thermo Fisher Scientific, Inc.) overnight at 4 °C, permeabilized with 70% (w/v) pure ethanol for 20 min minimum, and rinsed with 1X phosphate buffered saline solution, pH 7.4 (PBS, Fisher BioReagents Cat. BP665-1, Thermo Fisher Scientific) prior to immunostaining. .. The virus foci were immunostained using human monoclonal antibodies (hMAbs) 1.6D and D11C as previously described as primary tags in a 1X PBS solution with 0.1% (v/v) Tween 20 (Tween 20; Cat. 156054, Thermo Fisher Scientific Inc.), 5% (w/v) nonfat dry milk, and final antibody concentration of 1 μg/mL, rocked overnight at room temperature.

    Isolation:

    Article Title: Outcomes of autologous bone marrow mononuclear cell administration combined with educational intervention in the treatment of autism spectrum disorder: a randomized, open-label, controlled phase II clinical trial
    Article Snippet: .. In brief, the cells were isolated using density gradient centrifugation with Ficoll-PaqueTM PREMIUM density gradient media (Cytiva, Sweden) at 1400xg for 18 min, washed with 1x phosphate-buffered saline solution (Gibco, USA), and resuspended in 10 ml of autologous plasma. .. The total blood components before and after Ficoll‐Paque separation were evaluated by the laboratory department using a whole blood count.

    Gradient Centrifugation:

    Article Title: Outcomes of autologous bone marrow mononuclear cell administration combined with educational intervention in the treatment of autism spectrum disorder: a randomized, open-label, controlled phase II clinical trial
    Article Snippet: .. In brief, the cells were isolated using density gradient centrifugation with Ficoll-PaqueTM PREMIUM density gradient media (Cytiva, Sweden) at 1400xg for 18 min, washed with 1x phosphate-buffered saline solution (Gibco, USA), and resuspended in 10 ml of autologous plasma. .. The total blood components before and after Ficoll‐Paque separation were evaluated by the laboratory department using a whole blood count.

    Clinical Proteomics:

    Article Title: Outcomes of autologous bone marrow mononuclear cell administration combined with educational intervention in the treatment of autism spectrum disorder: a randomized, open-label, controlled phase II clinical trial
    Article Snippet: .. In brief, the cells were isolated using density gradient centrifugation with Ficoll-PaqueTM PREMIUM density gradient media (Cytiva, Sweden) at 1400xg for 18 min, washed with 1x phosphate-buffered saline solution (Gibco, USA), and resuspended in 10 ml of autologous plasma. .. The total blood components before and after Ficoll‐Paque separation were evaluated by the laboratory department using a whole blood count.



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